Tag: Protocols

Free Download Diagnostic Protocols in Endocrinology
English | 2024 | ISBN: 9811966524 | 195 Pages | PDF EPUB (True) | 15 MB
Endocrinology is perhaps, the only branch of medicine where stimulation and suppression testing are required as the initial step for biochemical confirmation of the clinical condition as hormone secretion is pulsatile. One of the primary needs for an endocrinologist is a pocketbook that can serve as a guide for performing and interpreting these tests. Unfortunately, there is no single source of these protocols, procedures, and interpretation of these tests in the country or even in the Southeast Asia region. This protocol manual is designed to be a ‘ready reckoner’ for endocrinologists for performing these tests.

Free Download Target Discovery and Validation Reviews and Protocols: Volume 2: Emerging Molecular Targets and Treatment Options By Mouldy Sioud, Marianne Leirdal (auth.), Mouldy Sioud (eds.)
2007 | 345 Pages | ISBN: 1588298906 | PDF | 7 MB
Target Discovery and Validation Reviews and Protocols, Volumes 1 and 2 review the most progressive and current methods for drug target discovery and validation. These volumes explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders.Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. The profound impact of genomics, proteomics and bioinformatics on target discovery is explored, and specific attention is given to the role of transgenic and knockout animals in functional genomics and target validation. Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. The methods and protocols collected here, all reviewed by leading scientists and clinicians, present the practical details necessary for translating the enormous discovery potential of the genome into real therapeutic products.Volume 2 collects all the practical details required for efficient translation of discovered targets into real pharmaceutical drugs. Specific targets in cancers and autoimmunity are described and the potential of using siRNAs, antisense oligonucleotides and RNA aptamers in patients is reviewed. This volume explores the tremendous impact of the application of genotyping and gene expression profiling on the future of healthcare, and presents cutting-edge protocols to aid in bringing agents against specific targets closer to application in the clinic.Collectively, these volumes provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application. For researchers, an understanding of available methods aids in the creation of innovative experiments in the laboratory, and the successful translation of target discovery to real therapeutics.

Free Download Placenta and Trophoblast: Methods and Protocols Volume 2 By Michael J. Soares, Joan S. Hunt (auth.), Michael J. Soares, Joan S. Hunt (eds.)
2006 | 452 Pages | ISBN: 1588296083 | PDF | 5 MB
Placental research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 2 provides readily reproducible protocols for studying trophoblast invasion, maternal immune cell responses to pregnancy, and the hormone-producing capabilities of the placenta. There are also methods to investigate the barrier function of the placenta, its role in regulating the transport of nutrients and wastes, and the adaptations of trophoblast cells and the placenta to disease. Highlights include techniques to analyze trophoblast cell interactions with the uterine vasculature and immune cells, and to the endocrine functions of the placenta. A companion first volume concentrates on methods for investigate embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.

Free Download Hypertension: Methods and Protocols By Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, Anna F. Dominiczak (auth.), Jérôme P. Fennell, Andrew H. Baker (eds.)
2005 | 502 Pages | ISBN: 1588293238 | PDF | 4 MB
Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.

Free Download Adoptive Immunotherapy: Methods and Protocols By David O’Neill, Nina Bhardwaj (auth.), Burkhard Ludewig, Matthias W. Hoffmann (eds.)
2005 | 500 Pages | ISBN: 1588294064 | PDF | 5 MB
Over the last decade, major advances in our understanding of the immune system and its mechanisms have led to promising new treatment strategies. In Adoptive Immunotherapy: Methods and Protocols, leading experts describe in detail their optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T-cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immonotherapy, and the utilization of regulatory T-cells. Theoretical overviews and discussion of future directions for the improvement of adoptive immunotherapy complete the volume. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Adoptive Immunotherapy: Methods and Protocols offers an authoritative collection of novel techniques to study the adoptive transfer of tailored antigen-specific immune cells and to improve their clinical application.

Free Download Fibrosis Research: Methods and Protocols By David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter (auth.), John Varga MD, David A. Brenner MD, Sem H. Phan MD, PhD (eds.)
2005 | 392 Pages | ISBN: 158829479X | PDF | 5 MB
Fibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.

Free Download Nanopore Sequencing : Methods and Protocols
by Kazuharu Arakawa
English | 2023 | ISBN: 1071629956 | 324 Pages | True ePUB | 39 MB

Free Download C++ Networking 101: Unlocking Sockets, Protocols, VPNs, and Asynchronous I/O with 75+ sample programs
by Anais Sutherland
English | 2023 | ISBN: 8119177126 | 274 Pages | ePUB | 0.13 MB

Free Download Higher-level WebRTC Protocols
Released 7/2023
MP4 | Video: h264, 1920×1080 | Audio: AAC, 44.1 KHz, 2 Ch
Genre: eLearning | Language: English | Duration: 10 Lessons ( 5h 37m ) | Size: 1.31 GB
The Higher-level WebRTC Protocols training course is meant to go deeper into how WebRTC works

Free Download WebRTC Courses – Low-level WebRTC Protocols
Released 1/2024
MP4 | Video: h264, 1920×1080 | Audio: AAC, 44.1 KHz, 2 Ch
Genre: eLearning | Language: English | Duration: 22 Lessons ( 5h 31m ) | Size: 2 GB
The Low-level WebRTC Protocols training course is focused on getting you up to a level of understand of the WebRTC protocols that will enable you to debug challenging network scenarios and how be able to approach implementing these protocols on your own if you choose to do so.